help  | about  | cite  | software

Pathway : DNA replication

Identifier  03030 Description  A complex network of interacting proteins and enzymes is required for DNA replication Generally, DNA replication follows a multistep enzymatic pathway At the DNA replication fork, a DNA helicase DnaB or MCM complex precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase pol III core, a sliding clamp beta, and a clamp loader gamma delta complex The DNA primase DnaG is needed to form RNA primers Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments In eukaryotes, three DNA polymerases alpha, delta, and epsilon have been identified DNA primase forms a permanent complex with DNA polymerase alpha PCNA and RFC function as a clamp and a clamp loader FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA

1 Data Sets

71 Genes

0 Proteins